GNM mobilities of the complete structure (left) and of the coarse-grained structure, every 40^th residue, (right) (a: head mode 1, b: head mode 2,   c: prohead mode 1, prohead mode 4).

  Introduction

   Theory & Methods

• We employed general elastic network analysis using: 

• Gaussian Network Model (GNM)

• Anisotropic Network Model (ANM)

• Additionally, due to the size of these structures  (~10⁵ residues), we employed two different application to the above elastic networks:

• Hierarchical Coarse-graining

• SIMPLE

   Results

• GNM fluctuations 

• ANM dynamics

   Conclusions

• Maturation proceeds by the covalent linking of a highly flexible residue (Lys169) to a highly constrained residue (Asn356), forming a stabilizing anchor.